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Updated: Sep 25, 2025

Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model
Published on: April 6, 2021
Classification of gases around Pseudomonas aeruginosa and Acinetobacter baumannii by infrared spectroscopy
Masato Yamamoto1, Satoru Arata2, Kunihiko Fukuchi3
1Department of Chemistry, College of Arts and Sciences, Showa University, 4562 Kamiyoshida, Fujiyoshida-city, Yamanashi 403-0005, Japan.
Abstract:
Gas samples were collected from the air surrounding single and mixed laboratory cultures, and preliminary data on human breath samples were also obtained. The infrared spectra of a variety of gasses were measured at high resolution (0.5 cm-1) to obtain information about the infrared absorption bands to be used as indicators. These key bands enable bacterial classification, and the discrimination rates can be improved by observing multiple infrared absorptions. The air around Pseudomonas aeruginosa was distinguished from the other gas samples by the infrared absorptions at wavenumbers of 778.4 cm-1 and 2213.2 cm-1. For Acinetobacter baumannii, infrared absorptions at 1215.0 cm-1 and 2982.3 cm-1 were used; furthermore, adding those at 4768.7 cm-1 and 5353.8 cm-1 was shown to improve identification.
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