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Retrospective confirmation of Mycoplasma infection by the immunoperoxidase technique
Abstract:
Rat tissues embedded in paraffin blocks stored for 8-10 yr were sectioned for immunoperoxidase staining. Sections of lung, trachea and endometrium of 22 of 32 rats that had exhibited clinical symptoms of mycoplasmal pneumonia prior to euthanasia, and whose organs had shown gross lesions suggesting mycoplasmal infection on necropsy, demonstrated the presence of Mycoplasma pulmonis organisms by the immunoperoxidase technique in one or more of these tissues.
Insights
Immunoperoxidase staining detected Mycoplasma pulmonis in tissues from rats with pneumonia symptoms. This technique confirmed the presence of the organism in lung, trachea, and endometrium tissues.
Area of Science:
- Veterinary Pathology
- Microbiology
- Immunohistochemistry
Background:
- Mycoplasma pulmonis is a significant respiratory pathogen in rats.
- Diagnosing mycoplasmal pneumonia can be challenging, especially in archived tissues.
Purpose of the Study:
- To evaluate the efficacy of the immunoperoxidase technique for detecting Mycoplasma pulmonis in archived rat tissues.
- To correlate the presence of Mycoplasma pulmonis with clinical signs and gross lesions.
Main Methods:
- Paraffin-embedded rat tissues (lung, trachea, endometrium) stored for 8-10 years were sectioned.
- Immunoperoxidase staining was performed to detect Mycoplasma pulmonis organisms.
Main Results:
- Mycoplasma pulmonis was detected in tissues from 22 of 32 rats exhibiting clinical signs and gross lesions suggestive of mycoplasmal pneumonia.
- The organism was identified in one or more of the examined tissues (lung, trachea, endometrium).
Conclusions:
- The immunoperoxidase technique is a viable method for detecting Mycoplasma pulmonis in long-term archived rat tissues.
- This study confirms the utility of immunohistochemistry in diagnosing past Mycoplasma pulmonis infections in rats.