Novel cloth-based closed bipolar solid-state electrochemiluminescence (CBP-SS-ECL) aptasensor for detecting...

Area of Science:

• Electrochemistry
• Biosensors
• Nanotechnology
• Biomedical Engineering

Background:

• Accurate detection of cancer biomarkers is crucial for early diagnosis and treatment monitoring.
• Existing biosensing platforms often face challenges with sensitivity, selectivity, and complex sample analysis.
• Closed bipolar electrochemistry (CBP) offers a unique platform for electrochemical sensing applications.

Purpose of the Study:

• To construct a novel cloth-based aptasensor for sensitive and selective detection of carcinoembryonic antigen (CEA).
• To integrate proximity hybridization (PH) and solid-state electrochemiluminescence (SS-ECL) within a CBP system.
• To evaluate the aptasensor's performance in detecting CEA in human serum samples and assess its potential for multiplexed detection.

Main Methods:

• Fabrication of cloth-based aptasensors using screen-printing.
• Integration of proximity hybridization (PH) and solid-state electrochemiluminescence (SS-ECL) in a closed bipolar electrochemistry (CBP) system.
• Immobilization of Ru(bpy)32+-doped silica nanoparticles (Ru-Si-NPs) on the closed bipolar electrode (C-BPE) anode.
• Utilizing aptamers and PH for target recognition and signal amplification.

Main Results:

• The aptasensor achieved quantitative detection of CEA within a linear range of 5-100000 pg mL−1.
• A low detection limit of 1.6 pg mL−1 was obtained, indicating high sensitivity.
• The sensor demonstrated acceptable sensitivity, selectivity, and stability, successfully detecting CEA in human serum.
• The aptasensor showed potential for simultaneous detection of CEA and K-ras gene.

Conclusions:

• The developed cloth-based CBP-SS-ECL aptasensor provides a sensitive, selective, and stable platform for CEA detection.
• The integration of PH and SS-ECL in a CBP system enhances electrochemiluminescence signals for improved detection.
• This versatile aptasensor shows promise for clinical diagnostics, including the potential for simultaneous detection of multiple biomarkers.