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Updated: Sep 25, 2025

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

GEM-IL: A highly responsive fluorescent lactate indicator.

Ramsey Bekdash^1,2,3, Jose R Quejada^1,2,3, Shunnosuke Ueno^1,2,4

¹Columbia Stem Cell Initiative, Columbia University, New York, NY 10032, USA.

Cell Reports Methods

|April 27, 2022

Summary

This summary is machine-generated.

Researchers developed a new tool, genetically encoded metabolic indicator for probing lactate (GEM-IL), to detect lactate changes in live cells. This advancement offers improved specificity and sensitivity for studying cellular lactate dynamics in health and disease.

Keywords:

cancer cardiac metabolism cellular metabolism genetically encoded fluorescent indicators lactate metabolite indicator

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Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

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Published on: October 15, 2018

Area of Science:

Cellular metabolism and signaling
Biomedical engineering and biosensing

Background:

Lactate metabolism plays critical roles in cellular functions, disease development, and pathophysiology.
Existing methods for detecting lactate changes in live cells are limited, driving the need for novel approaches.

Purpose of the Study:

To develop a novel genetically encoded metabolic indicator for real-time detection of lactate.
To enhance specificity and sensitivity in probing lactate dynamics within live cells.

Main Methods:

Development of a genetically encoded metabolic indicator for probing lactate (GEM-IL) using superfolder fluorescent proteins and mutagenesis.
Optimization of the indicator's design for improved specificity and sensitivity.
Demonstration of GEM-IL functionality in human oncogenic neural progenitor cells and mouse primary ventricular myocytes.

Main Results:

Successful development of GEM-IL, a novel genetically encoded lactate indicator.
GEM-IL demonstrates enhanced specificity and sensitivity compared to previous lactate indicators (e.g., Laconic, Green Lindoblum).
GEM-IL effectively detected lactate changes in distinct cell types, including human oncogenic neural progenitor cells and mouse primary ventricular myocytes.

Conclusions:

The developed GEM-IL provides a powerful new tool for investigating lactate dynamics in live cells.
GEM-IL has the potential to significantly advance our understanding of lactate's roles in cellular physiology and disease.
This metabolic indicator opens new avenues for research in cellular metabolism and disease pathophysiology.