Light and scanning electron microscopy observations on morphological diversity of Rosaceae stigma and its taxonomic significance

The stigma morphology can provide a reference for exploring plant systematics and pollination biology. In this study, we observed the stigma morphological characteristics of Rosaceae in Beijing urban area in detail using light microscopy and scanning electron microscopy. The stigma of Rosaceae is entire or bilobed and mostly baculate, crateriform, cristate, discoid, or flattened. The stigma surface may have irregular, strongly raised ridges; or flat without papillae; or composed of densely or loosely arranged papillary cells. Surface ornamentation includes fossulate, psilate, psilate-striate, rugulate, scabrate, striate, and striate-rugulate. There are similarities in stigma morphology among genera and differences in stigma morphology among species within genera. The stigma shape supports the view of molecular systematic classification, that is, the former subfamilies Maloideae, Prunoideae, and Spiraeoideae are grouped into subfamily Amygdaloideae. RESEARCH HIGHLIGHTS: Scanning electron microscopy (SEM) was used to provide high-quality figures for observing stigma morphology. The data on the morphological diversity of stigma were provided to further explore the systematics and pollination biology of Rosaceae.