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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Non-invasive Raman spectroscopy for time-resolved in-line lipidomics.

Karin Wieland1,2, Mahmoud Masri3, Jeremy von Poschinger4

  • 1Chair of Analytical Chemistry, Technical University of Munich Elisabeth-Winterhalter-Weg 6 81377 Germany karin.wieland@tum.de haisch@tum.de.

RSC Advances
|April 28, 2022
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Summary
This summary is machine-generated.

Raman spectroscopy offers a faster, non-invasive method for monitoring lipid production in oil-producing yeast. This technique enables real-time process control for sustainable palm oil alternatives.

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Area of Science:

  • Biotechnology
  • Spectroscopy
  • Industrial Microbiology

Background:

  • Oil-producing yeasts offer a sustainable alternative to palm oil.
  • Accurate lipid composition analysis is vital for optimizing yeast fermentation processes.
  • Current off-line methods like gas chromatography are time-consuming and cumbersome.

Purpose of the Study:

  • To introduce non-invasive in-line Raman spectroscopy as a rapid method for yeast lipidomics.
  • To demonstrate the potential of Raman spectroscopy for real-time process control in oleaginous yeast fermentation.
  • To correlate spectroscopic data with biomass, lipid production, and glucose consumption.

Main Methods:

  • Fed-batch fermentation of the oleaginous yeast *C. oleaginosus*.
  • Application of in-line Raman spectroscopy for non-invasive monitoring.
  • Development of Partial Least Squares (PLS) regression models for data analysis.
  • Gaussian curve fitting to analyze fatty acid trends.

Main Results:

  • Raman spectroscopy successfully monitored biomass formation, lipid production, and glucose consumption in real-time.
  • PLS-regression models provided timely, process-relevant information.
  • Analysis revealed trends in saturated and unsaturated fatty acid production during fermentation.

Conclusions:

  • Raman spectroscopy is a viable and efficient tool for in-line lipidomics in oleaginous yeasts.
  • This method facilitates faster process control for improved yield and quality.
  • It supports the development of sustainable biotechnological processes for oil production.