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Biosynthesis of Polysaccharides

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Polysaccharide from rubescens: extraction, optimization, characterization and antioxidant activities.

Shuang Cheng¹, Fei He¹, Longyang Fu¹

¹School of Chemical Engineering, Zhengzhou University Zhengzhou 450001 China.

|April 28, 2022

Summary

Researchers optimized rubescens polysaccharide extraction using response surface methodology (RSM). The study identified optimal conditions and confirmed the polysaccharide

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Area of Science:

Biochemistry
Natural Product Chemistry

Background:

Rubescens is recognized for anti-tumor properties, prompting investigation into its other bioactive compounds.
Polysaccharides are known for diverse biological activities, including antioxidant effects.
Optimizing extraction is crucial for maximizing yield and preserving bioactivity.

Purpose of the Study:

To extract and optimize the yield of rubescens polysaccharide.
To elucidate the preliminary structure of the extracted polysaccharide.
To evaluate the antioxidant activity of rubescens polysaccharide.

Main Methods:

Response surface methodology (RSM) was employed for optimizing extraction conditions.
High-Performance Liquid Chromatography (HPLC), X-ray diffraction, and Fourier-Transform Infrared Spectroscopy (FT-IR) were used for structural analysis.
In vitro antioxidant assays (DPPH, ABTS, hydroxyl radical scavenging) were conducted.

Main Results:

Optimal extraction conditions determined: solvent-solid ratio 33.33:1, temperature 87.70 °C, time 2.59 h, yielding 11.92%.
Polysaccharide composition: Mannose:Glucose:Galactose:Xylose:Arabinose ratio of 2.27:4.65:2.57:1:1.85.
Rubescens polysaccharide demonstrated significant reducing capacity and scavenging activity against DPPH, ABTS, and hydroxyl radicals.

Conclusions:

Optimized hot-water extraction provides an efficient method for obtaining rubescens polysaccharide.
The extracted rubescens polysaccharide exhibits notable antioxidant properties, suggesting potential as a natural antioxidant source.
This research supports the broader utilization of rubescens resources for developing novel therapeutic agents.