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Updated: Sep 25, 2025
A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
Published on: December 23, 2016
Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations
M Teresa de Martino1, Fabio Tonin2, Victor R L J Bloemendal1,3
1Department of Chemical Engineering & Chemistry, Institute for Complex Molecular Systems, Eindhoven University of Technology PO Box 513 5600 MB Eindhoven The Netherlands J.C.M.v.Hest@tue.nl.
Abstract:
A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1) N-acetylneuraminate lyase (NAL) is internalized in NAL-c-CLEnAs and used in a continuous flow aldol condensation of N-acetyl-d-mannosamine with sodium pyruvate to N-acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases (HSDH) 7α- and 7β-HSDH are incorporated in c-CLEnAs and used in a two-step cascade batch synthesis of ursodeoxycholic acid (UDCA). The versatile use of c-CLEnA demonstrates that this immobilization methodology is a valuable addition to the toolbox of synthetic chemists.
