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Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Materials Science

Background:

  • Electrochemical biosensors are valuable for low-resource diagnostics due to ease of use and low-cost instrumentation.
  • Disposable gold screen-printed electrodes (SPEs) are common substrates but can suffer from dopant interference and heterogeneity.
  • An alternative fabrication method using gold leaf offers a potentially superior disposable electrode.

Purpose of the Study:

  • To compare the surface topology, biorecognition element deposition, and functional performance of gold leaf electrodes against commercial SPEs.
  • To evaluate the suitability of gold leaf electrodes for reproducible and effective biosensing.
  • To assess the cost-effectiveness of gold leaf electrodes for potential point-of-care applications.

Main Methods:

  • Fabrication of disposable gold electrodes from gold leaf using a low-cost, equipment-free method.
  • Direct comparison of gold leaf electrodes with two commercial SPEs.
  • Characterization of surface topology and biorecognition element deposition.
  • Evaluation of functional performance using a DNase I assay.

Main Results:

  • Gold leaf electrodes demonstrated superior performance in reproducible and effective biosensing compared to SPEs.
  • Significant differences in surface topology and biorecognition element deposition were observed.
  • Gold leaf electrodes are nearly an order of magnitude less expensive than commercial SPEs.

Conclusions:

  • The developed gold leaf electrodes significantly outperform commercial SPEs for biosensing applications.
  • These electrodes offer a cost-effective and high-performance alternative for disposable electrochemical biosensors.
  • Gold leaf electrodes show great promise for the development of point-of-care diagnostics, particularly in low-resource environments.