Related Experiment Video

Updated: Oct 3, 2026

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
15:16

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

17.3K

Molecular Detection of Enteric Viruses in Under-Five Children with Diarrhea in Debre Tabor, Northwest Ethiopia

Aschalew Gelaw1,2, Uwe G Liebert2

  • 1Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences University of Gondar, Gondar, Ethiopia.

Infection and Drug Resistance
|April 28, 2022
PubMed

Insights

Viral gastroenteritis is a major health issue for children globally, especially in Sub-Saharan Africa. This study found diverse enteric viruses, including human adenovirus and noroviruses, in children with diarrhea, highlighting the need for further research.

Area of Science:

  • Virology
  • Public Health
  • Pediatrics

Background:

  • Viral gastroenteritis poses a significant global public health challenge, particularly impacting infants and children.
  • Sub-Saharan Africa experiences the highest burden of morbidity and mortality associated with viral gastroenteritis.

Purpose of the Study:

  • To determine the prevalence and genetic diversity of enteric viruses in children suffering from diarrhea.
  • To assess the etiological agents contributing to diarrheal diseases in pediatric populations.

Main Methods:

  • A cross-sectional study was conducted involving 38 children with diarrhea in Debre Tabor.
  • Fecal samples were analyzed using reverse transcription-polymerase chain reaction (RT-PCR) to detect enteric viruses.
  • Data analysis involved descriptive statistics using SPSS software.

Main Results:

  • Over half (52.6%) of the children tested positive for at least one enteric virus.
  • Human adenovirus (HAdV) was the most prevalent (18.4%), followed by noroviruses (NoVs) and enteroviruses (EV) (13.2% each).
  • Nineteen distinct enteric virus genotypes were identified, including diverse strains of adenovirus, norovirus, sapovirus, rotavirus A, and enterovirus.

Conclusions:

  • The study identified a wide range of enteric viruses in young children experiencing diarrhea.
  • The significant genetic diversity observed underscores the need for larger, multicenter studies to fully understand the burden and diversity of enteric viruses.
Abstract

Keywords:
Debre TaborEthiopiadiarrheaenteric virusesunder-five children

More Related Videos

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay11:09

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay

Published on: September 11, 2016

8.9K
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR12:32

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

Published on: January 16, 2016

12.9K

Related Concept Videos

Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...
Poliomyelitis01:17

Poliomyelitis

Poliomyelitis is caused by poliovirus, a small, non-enveloped, positive-sense RNA virus of the Picornaviridae family and Enterovirus genus. Transmission occurs primarily via the fecal-oral route, often through ingestion of contaminated water or food. The virus initially replicates in the oropharynx and intestinal mucosa, particularly in lymphoid tissues such as the tonsils, Peyer’s patches, and regional lymph nodes. Primary viremia follows, allowing dissemination throughout the body.In most...