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Epoxide hydrolase expression in human and rodent established cell lines
Xenobiotica; the Fate of Foreign Compounds in Biological Systems
|January 1, 1987
Summary
This study analyzed epoxide hydrolase activity in human, hamster, and mouse cell lines, comparing it to tissue preparations. Enzyme characterization using antibodies revealed species-specific differences, suggesting evolutionary divergence.
Area of Science:
- Biochemistry
- Enzymology
- Comparative biology
Background:
- Epoxide hydrolases are crucial enzymes involved in xenobiotic metabolism and detoxification.
- Understanding their activity across different species and cell types is important for toxicological and pharmacological studies.
Purpose of the Study:
- To analyze and compare epoxide hydrolase activity in established human, hamster, and mouse cell lines.
- To characterize the epoxide hydrolase enzymes in these cell lines using species-specific antibodies.
Main Methods:
- Epoxide hydrolase activity was measured using styrene oxide as a substrate.
- Enzyme characterization was performed using antibodies against human and mouse liver epoxide hydrolase.
Main Results:
- Cell lines exhibited a wide range of epoxide hydrolase activities (0.005–0.44 nmol/min per mg protein).
- Human and mouse tissue preparations showed significantly higher activities (12.2 and 2.7 nmol/min per mg protein, respectively).
- Antibody-based characterization indicated distinct enzyme profiles across species, with cross-reactivity observed in hamster cell lines.
Conclusions:
- Epoxide hydrolase activity varies considerably among human, hamster, and mouse cell lines.
- Immunological data suggest evolutionary differences in epoxide hydrolase enzymes among these species.