[A stepwise method of evaluating sugar substitutes--a preliminary study using enzymes. 2. Beta-fructosidase from...

No D-glucosylfructoses except sucrose, neither the alpha(1----1)- nor the alpha(1----3)- or the alpha(1----5)- or the alpha(1----6)-disaccharide possess substrate properties for beta-fructosidase from yeast. The two latter ones, leucrose and isomaltulose, however, are non-competitive (leucrose) or uncompetitive (isomaltulose, Palatinose) inhibitors of beta-fructosidase from yeast. Due to the high substrate specificity of invertase, assays of its activity have predictive power for cariological aspects of sugar substitutes carrying glycoside bonds between glucose and fructose.