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Updated: Sep 25, 2025
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
Development of an ultrafast fluorescent probe for specific recognition of hypochlorous acid and its application in
Zhencai Xu1,2, Xiaofeng Wang1, Tingting Duan1
1Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Hainan Medical University Haikou 570102 China xuejunzhouent@hainmc.edu.cn.
Abstract:
Hypochlorous acid (HOCl), a highly potent oxidant of reactive oxygen species, plays critical roles in many physiological and pathological processes. In this work, a novel coumarin-based fluorescent probe, Cou-HOCl, was prepared for the detection of HOCl. The probe exhibited good selectivity over other analytes, excellent sensitivity with a detection limit of 16 nM, and fast response within 5 s. And further study demonstrated that the probe could be used not only to image exogenous HOCl in various cells, but also to determine the fluctuating levels of HOCl in macrophage cells during inflammation.
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