Supported Pt Enabled Proton-Driven NAD(P)⁺ Regeneration for Biocatalytic Oxidation

Area of Science:

• Catalysis
• Biochemistry
• Materials Science

Background:

• Biocatalytic oxidations are crucial for chemical synthesis but are limited by expensive nicotinamide adenine dinucleotide (NAD(P)⁺) cofactor dependency.
• Efficient regeneration of NAD(P)⁺ from its reduced form, NAD(P)H, is essential for the commercial viability of these processes.

Purpose of the Study:

• To design and develop a heterogeneous catalyst for efficient NAD(P)⁺ regeneration.
• To investigate the application of this catalyst in supporting biocatalytic alcohol oxidation reactions.

Main Methods:

• Development of carbon-supported platinum (Pt) catalysts with modified carbon supports to tune electronic properties.
• Proton-driven oxidation of NAD(P)H to NAD(P)⁺ with concurrent hydrogen formation using the Pt catalysts.
• Testing the compatibility and performance of the heterogeneous Pt catalyst in enzymatic oxidation of various alcohols.

Main Results:

• Electron-rich Pt nanoparticles on a modified carbon support demonstrated the highest catalytic activity for NAD(P)⁺ regeneration (TOF = 581 h^-1).
• The heterogeneous Pt catalyst showed excellent compatibility with alcohol dehydrogenases in biocatalytic systems.
• Successful conversion of alcohols to ketones or lactones was achieved, supported by the NAD(P)⁺ regeneration system.

Conclusions:

• A cooperative inorganic-enzymatic catalytic system using Pt catalysts for NAD(P)⁺ regeneration is a promising strategy for chemical synthesis.
• This approach overcomes the limitations of expensive cofactor dependency in biocatalytic NAD(P)⁺-dependent pathways.
• The developed catalyst enables efficient and sustainable biocatalytic oxidations.