Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Overhauser Enhancement (NOE)01:07

Nuclear Overhauser Enhancement (NOE)

862
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling.  This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
862
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.2K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.2K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

559
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
559
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

326
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
326
Atomic Nuclei: Nuclear Spin State Population Distribution01:14

Atomic Nuclei: Nuclear Spin State Population Distribution

1.2K
Near absolute zero temperatures, in the presence of a magnetic field, the majority of nuclei prefer the lower energy spin-up state to the higher energy spin-down state. As temperatures increase, the energy from thermal collisions distributes the spins more equally between the two states. The Boltzmann distribution equation gives the ratio of the number of spins predicted in the spin −½ (N−) and spin +½ (N+) states.
1.2K
Atomic Nuclei: Magnetic Resonance01:05

Atomic Nuclei: Magnetic Resonance

779
The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
779

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Solid-state structure and supra-molecular packing of 1,2-bis-(2-phenyl-1<i>H</i>-benzo[<i>d</i>]imidazol-1-yl)diazene.

Acta crystallographica. Section E, Crystallographic communications·2026
Same author

Labilized ligands under ultra-confinement: a solid-state <sup>2</sup>H NMR investigation of Ln(BH<sub>4</sub>)<sub>2</sub>(THF)<sub>2</sub>/HY<sub>30</sub>.

Chemical communications (Cambridge, England)·2026
Same author

Group 4 Metallocenes Supported on Sulfated Zirconium Oxide Catalyze Benzene C-H Borylation.

Journal of the American Chemical Society·2026
Same author

3<i>H</i>-Phenothiazin-3-one: a photocatalyst for the mild oxidation of boronic acids under green LED irradiation.

Organic & biomolecular chemistry·2026
Same author

Hydration and transport properties of cesium hydroxide and mixed cesium hydroxide-sodium nitrite aqueous solutions.

The Journal of chemical physics·2026
Same author

Solvation and Cation Competition in Mixed Hydroxide Brines.

The journal of physical chemistry. B·2026
Same journal

Precursor-Directed Self-Assembly in Hydrothermal Carbon Nitride Nanostructures Revealed by Nano-FTIR.

The journal of physical chemistry letters·2026
Same journal

Correction to "Equation-of-Motion Block-Correlated Coupled Cluster Method for Excited Electronic States of Strongly Correlated Systems".

The journal of physical chemistry letters·2026
Same journal

Rationalizing Stacking-Dependent Charge Injection Dynamics in Radical-Based Organic Light-Emitting Diodes.

The journal of physical chemistry letters·2026
Same journal

Bottom-Up Formation of the Simplest Geminal Thiol─Methanedithiol (CH<sub>2</sub>(SH)<sub>2</sub>)─and the Methyl Hydrodisulfide (H<sub>3</sub>CSSH) Isomer in Interstellar Analogue Ices.

The journal of physical chemistry letters·2026
Same journal

Trion Mediated Sequential Charge Separation in Functionalized CsPbBr<sub>3</sub>/AgInS<sub>2</sub> Hybrid Nanocrystals.

The journal of physical chemistry letters·2026
Same journal

Linking Local Water Electrostatic Potentials to Measured Hydrogen Evolution Onset in Aqueous Electrolytes.

The journal of physical chemistry letters·2026
See all related articles

Related Experiment Video

Updated: Sep 25, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.6K

Methyl-Driven Overhauser Dynamic Nuclear Polarization.

Frédéric A Perras1, Dragos F Flesariu2, Scott A Southern1

  • 1US DOE, Ames Laboratory, Ames, Iowa 50011, United States.

The Journal of Physical Chemistry Letters
|April 28, 2022
PubMed
Summary
This summary is machine-generated.

The Overhauser effect in solids can be triggered by methyl group rotation, which modulates hyperfine coupling. This finding enables the design of novel polarizing agents for dynamic nuclear polarization (DNP) applications.

More Related Videos

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

10.8K
Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

7.6K

Related Experiment Videos

Last Updated: Sep 25, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.6K
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

10.8K
Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

7.6K

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR)
  • Dynamic Nuclear Polarization (DNP)

Background:

  • The Overhauser effect is a key dynamic nuclear polarization (DNP) mechanism, typically requiring mobile unpaired electrons.
  • In insulating solids, Overhauser DNP necessitates structural modulations that alter hyperfine coupling.

Purpose of the Study:

  • To investigate if Overhauser DNP can be induced in insulating solids via controlled structural modulations.
  • To demonstrate the role of methyl group rotation in modulating hyperfine interactions for Overhauser DNP.

Main Methods:

  • Utilized a Blatter's radical as a model system.
  • Introduced methyl groups to modulate hyperfine coupling.
  • Employed deuteration to remove methyl protons and assess their impact.
  • Varied hyperfine coupling strength to observe its effect on DNP.

Main Results:

  • Methyl group rotation in Blatter's radical successfully triggered Overhauser DNP in insulating solids.
  • Deuteration of methyl protons quenched the Overhauser DNP signal.
  • Reduced hyperfine coupling strength also diminished the Overhauser DNP effect.

Conclusions:

  • Methyl group rotation is an effective strategy to modulate hyperfine interactions and facilitate Overhauser DNP in solids.
  • This mechanism opens avenues for designing custom Overhauser DNP polarizing agents for high-field magic angle spinning (MAS-DNP).