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Published on: December 29, 2015
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Loquacious modulates flaviviral RNA replication in mosquito cells
Shwetha Shivaprasad1, Kuo-Feng Weng1, Yaw Shin Ooi1
1Department of Microbiology & Immunology, Stanford University SOM, Stanford, California, United Sates of America.
Plos Pathogens
|April 28, 2022
Summary
Researchers identified mosquito proteins Sec61A1 and Loquacious (Loqs) that interact with dengue virus RNA. Depleting these proteins impacted viral replication, suggesting they have pro-viral functions in mosquito hosts.
Area of Science:
- Virology
- Molecular Biology
- Entomology
Background:
- Arthropod-borne viruses infect mosquitoes and mammals.
- Virus-host interactions in mammalian hosts are well-studied.
- Mosquito host interactions, especially inhibitory strategies, are less understood.
Purpose of the Study:
- To identify mosquito proteins interacting with dengue viral RNA.
- To investigate the role of these proteins in the viral infectious cycle.
Main Methods:
- Novel RNA-Protein interaction detection assay used in infected mosquito cells.
- Depletion analysis to assess protein function.
- Co-localization and pull-down assays to confirm interactions.
Main Results:
- Membrane-associated proteins Sec61A1 and Loquacious (Loqs) were found complexed with dengue viral RNA.
- Depletion of Sec61A1 and Loqs demonstrated pro-viral functions.
- Loqs interacts with viral protein NS3 and viral RNAs, modulating viral replication.
Conclusions:
- Sec61A1 and Loqs are key mosquito proteins involved in the dengue viral infectious cycle.
- Loqs plays a conserved pro-viral role in flavivirus infections within mosquito cells.
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