Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of potent, selective cPLA<sub>2</sub> inhibitors for targeting neuroinflammation in Alzheimer's disease and other neurodegenerative disorders.

npj drug discovery·2026
Same author

Systematic Characterization of LUHMES Cell-Based Parkinson's Disease Models Reveals Potential Novel Drug Targets.

Molecular neurobiology·2025
Same author

Biocompatible PLA/<i>spirulina</i> microparticles via electrospraying for targeted drug delivery in HUVEC and HaCaT cell lines.

Journal of biomaterials science. Polymer edition·2025
Same author

The Role of Pea3 Transcription Factor Subfamily in the Nervous System.

Molecular neurobiology·2024
Same author

Mitotic Kinases Aurora-A, Plk1, and Cdk1 Interact with Elk-1 Transcription Factor through the N-Terminal Domain.

International journal of cell biology·2024
Same author

Multiple parallel cell lineages in the developing mammalian cerebral cortex.

Science advances·2024

Related Experiment Video

Updated: Sep 25, 2025

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research
05:55

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research

Published on: December 1, 2023

974

Multiple Sclerosis Biomarker Candidates Revealed by Cell-Type-Specific Interactome Analysis.

Kübra Yurduseven1,2, Yigit Koray Babal1, Esref Celik2

  • 1Institute of Biotechnology, Gebze Technical University, Kocaeli, Turkey.

Omics : a Journal of Integrative Biology
|April 28, 2022
PubMed
Summary

This study reveals cell-specific interactions in multiple sclerosis (MS) brain tissue, identifying key proteins and potential blood biomarkers for disease subtypes. These findings offer new diagnostic and therapeutic avenues for MS.

Keywords:
biomarkersinteractomemolecular targetsmultiple sclerosisneurodegenerative diseasespersonalized medicine

More Related Videos

Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice
08:49

Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice

Published on: October 6, 2023

2.0K
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
07:57

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

8.6K

Related Experiment Videos

Last Updated: Sep 25, 2025

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research
05:55

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research

Published on: December 1, 2023

974
Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice
08:49

Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice

Published on: October 6, 2023

2.0K
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
07:57

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

8.6K

Area of Science:

  • Neuroscience
  • Bioinformatics
  • Systems Biology

Background:

  • Multiple sclerosis (MS) is a central nervous system demyelinating disease with significant patient variability.
  • Current biomarkers for predicting MS clinical phenotypes and outcomes are limited.
  • Cell-type-specific interactome analysis presents a promising approach for novel diagnostics and therapeutics in MS.

Purpose of the Study:

  • To identify cell-type-specific interaction networks in the white matter (WM) and gray matter (GM) of the brain in MS.
  • To discover differentially regulated hub proteins critical for MS pathogenesis.
  • To identify potential blood-based biomarkers for different MS subtypes through comparative transcriptomic analysis.

Main Methods:

  • Bioinformatic analysis of MS datasets.
  • Cell-type-specific interactome profiling in WM and GM.
  • Comparative analysis of brain and peripheral blood transcriptomics.

Main Results:

  • WM interactions in MS are oligodendrocyte-focused, while GM interactions are primarily neuron-centric.
  • Key differentially regulated hub proteins identified include APP, EGLN3, PTEN, and LRRK2.
  • Potential biomarker candidates (NRGN, CRTC1, CDC42, IFITM3) were identified through brain-blood comparative analysis.

Conclusions:

  • Cell-type-specific interactome analysis provides a unique network view of MS pathogenesis.
  • The study identifies potential biomarkers for MS subtypes by comparing brain and blood transcriptomics.
  • This systems science approach holds promise for understanding other neurodegenerative disorders.