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Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Electrophoresis: Overview01:20

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Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
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Ultra-sensitive dielectrophoretic surface charge multiplex detection inside a micro-dielectrophoretic device.

Kang In Yeo1, Insu Park2, Sang Hyun Lee1

  • 1Department of Biomedical Engineering, Yonsei University, Wonju, 26493, Republic of Korea.

Biosensors & Bioelectronics
|April 28, 2022
PubMed
Summary

This study introduces a novel method for detecting multiple metal ions using optical discrimination of microparticle probes. The technique enables sensitive, label-free, multiplex detection of mercury and silver ions in drinking water.

Keywords:
DielectrophoresisLabel freeMicro-dielectrophoretic deviceMultiplex detectionSurface chargeUltra-sensitive

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Area of Science:

  • Analytical Chemistry
  • Nanotechnology
  • Biomolecular Engineering

Background:

  • Label-free dielectrophoretic (DEP) force-based surface charge detection offers high sensitivity for detecting analytes.
  • Current DEP methods face challenges in multiplex detection due to complex calibration and signal interference.

Purpose of the Study:

  • To develop a novel method for overcoming limitations in multiplex dielectrophoretic detection.
  • To enable sensitive and selective label-free detection of multiple analytes simultaneously.

Main Methods:

  • Developed a method using optical discrimination of dielectrophoretic behaviors of multiple microparticle probes.
  • Utilized surface charge differences before and after self-assembling conjugation of probes.
  • Functionalized particle probes with DNA aptamers for specific analyte binding.

Main Results:

  • Achieved attomolar limit of detection (LOD) for Hg2+ in distilled water and femtomolar LOD in drinking water using DNA aptamer-functionalized probes.
  • Demonstrated the first label-free dielectrophoretic multiplex detection of Hg2+ and Ag+ in drinking water.
  • Attained femtomolar LOD for Hg2+ and nanomolar LOD for Ag+ in multiplex detection.

Conclusions:

  • The developed optical discrimination method enhances multiplex dielectrophoretic detection capabilities.
  • This technique provides a sensitive and selective platform for label-free simultaneous detection of multiple targets.
  • Opens new avenues for environmental monitoring and diagnostics.