Effects of tetramethyl bisphenol F on thyroid and growth hormone-related endocrine systems in zebrafish larvae

Trimethyl bisphenol F (TMBPF) has recently been used as a bisphenol A substitute in polymer coatings for metal cans containing beverages or food. This study investigated whether TMBPF disrupts the endocrine system associated with thyroid hormones and growth hormones employing zebrafish embryos and larvae. After 14 days of exposure, body weight was significantly reduced when zebrafish were exposed to a TMBPF concentration greater than 50 μg/L. The triiodothyronine levels were significantly increased, while growth hormone levels were significantly decreased in larvae exposed to 5 μg/L TMBPF. The transcription of genes associated with thyroid hormone production (trα, tpo, tg, and nis), deiodination (deio2), growth hormone production (gh1, ghrh, and ghra), and insulin-like growth factor (igf2a, igf2b, igf2r, igfbp1a, igfbp1b, igfbp2a, igfbp2b, and igfbp5a) was significantly upregulated, whereas the transcription of genes association with thyrotropin-releasing hormone (trh and trhr1) was significantly downregulated. These results suggest that hyperthyroidism, decrease in growth hormone, and regulation of genes involved in the hypothalamus-pituitary-thyroid and growth hormone/insulin-like growth factor might be responsible for the observed growth inhibition in larvae exposed to TMBPF. The bioaccumulation of TMBPF and its effects on the endocrine system after chronic exposure requires further investigation.