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A double-blind comparative immunotypic study between two institutions phenotyping non-Hodgkin's lymphomas.

T M Grogan, R R Tubbs

American Journal of Clinical Pathology

|April 1, 1987

Summary

This study shows that immunophenotyping lymphoma specimens after long-distance air transport is feasible. High reproducibility (93%) was achieved, supporting collaborative lymphoma research between institutions.

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Area of Science:

Hematology
Immunology
Pathology

Background:

Accurate immunophenotyping is crucial for lymphoma diagnosis and subtyping.
Long-distance specimen transport can potentially compromise sample integrity and diagnostic accuracy.

Purpose of the Study:

To assess the feasibility and reproducibility of immunophenotyping lymphoma specimens after air express transport between institutions.
To evaluate the impact of transport on B-cell and T-cell antigen expression.

Main Methods:

39 lymphoma cases (26 B-cell, 13 T-cell) were air-expressed as snap-frozen sections on slides.
Immunohistochemistry was performed using a common panel of 20 antibodies against B- and T-cell antigens.
Specimens were assayed before and after transport in a double-blind, paired determination fashion.

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Main Results:

Overall agreement between pre- and post-transport immunophenotyping was 93.3% (624/669 determinations).
Interinstitutional agreement for T-cell antigens was 94% and for B-cell antigens was 93%.
Despite minor discrepancies in specific antigens, the overall phenotypic profile (B vs. T, subset stage) was consistent for all 39 cases.

Conclusions:

Long-distance air transport of snap-frozen lymphoma sections is a feasible method for immunophenotyping.
High reproducibility in immunophenotyping supports the potential for multi-institutional collaborative lymphoma studies.
Minor discrepancies observed may be attributed to antigen loss, reagent variability, or methodological differences, but do not affect overall diagnostic classification.