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Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

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Postcolumn Infusion as a Quality Control Tool for LC-MS-Based Analysis.

Oskar González¹, Anne-Charlotte Dubbelman¹, Thomas Hankemeier¹

¹Analytical BioSciences and Metabolomics, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.

Journal of the American Society for Mass Spectrometry

|April 28, 2022

Summary

This summary is machine-generated.

Continuous postcolumn infusion monitoring enhances liquid chromatography-mass spectrometry (LC-MS) quality control. This method reliably detects analytical flaws and matrix effects in bioanalysis, improving method performance.

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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

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Published on: April 26, 2016

Area of Science:

Analytical Chemistry
Biochemistry

Background:

Postcolumn infusion is a technique used to study matrix effects in liquid chromatography-mass spectrometry (LC-MS).
Currently, it is primarily utilized during method development and validation phases.

Purpose of the Study:

To demonstrate the value of continuous postcolumn infusion for ongoing quality monitoring of LC-MS analyses.
To highlight its utility in identifying performance issues and unexpected matrix effects.

Main Methods:

A transferable protocol for continuous postcolumn infusion was developed.
The protocol was applied to real-world bioanalytical scenarios.

Main Results:

Continuous postcolumn infusion effectively monitored LC-MS analysis quality.
The method successfully detected flaws in analytical performance.
Unexpected sources of matrix effects were identified, aiding in sample treatment evaluation.

Conclusions:

Continuous postcolumn infusion is a valuable tool for routine quality control in LC-MS.
This approach facilitates early detection of method deviations and matrix effect issues in bioanalysis.