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Evaluation of the Potential Probiotic Yeast Characteristics with Anti-MRSA Abilities
Yong Shen1, Xue Bai1, Yan Zhang1
1College of Food Science and Engineering, Jilin University, Changchun, 130062, People's Republic of China.
Abstract:
Methicillin-resistant Staphylococcus aureus (MRSA) is a disreputable pathogenic bacterium that has been proven to colonize the intestinal tract. The goal of this study is to find anti-MRSA probiotic yeast from food and evaluate its probiotic characteristics and safety. Finally, 15 strains were isolated from fruit peel with anti-MRSA ability. Using DNA sequence analysis, they were identified as the genus Hanseniaspora (7 strains) and Starmerella (8 strains). Starmerella bacillaris CC-PT4 (CGMCC No. 23573) that was isolated from the grape peel has good auto-aggregation ability and hydrophobicity, and can tolerate 0.3% bile, pH 2, simulated gastric fluid (SGF), and simulated intestinal fluid (SIF). Strikingly, Starmerella bacillaris CC-PT4, like commercial probiotic Saccharomyces boulardii CNCM I-745 (Florastor ®), can adapt to the temperature of the human body (37 ℃). After safety assessment, this strain is sensitive to amphotericin B and cannot produced β-hemolytic activities. Overall, this study provides a new candidate for probiotic yeast with anti-MRSA ability.
Insights
Researchers discovered a new probiotic yeast, Starmerella bacillaris CC-PT4, from grape peel effective against Methicillin-resistant Staphylococcus aureus (MRSA). This yeast exhibits beneficial probiotic traits and safety, offering a potential new strategy against MRSA colonization.
Area of Science:
- Microbiology
- Food Science
- Probiotics
Background:
- Methicillin-resistant Staphylococcus aureus (MRSA) is a significant pathogen colonizing the human gut.
- There is a need for novel probiotic agents to combat MRSA infections.
Purpose of the Study:
- To isolate and identify food-derived probiotic yeasts with anti-MRSA activity.
- To evaluate the probiotic characteristics and safety of promising anti-MRSA yeast strains.
Main Methods:
- Isolation of yeast strains from fruit peels with anti-MRSA activity.
- Identification of isolates using DNA sequencing (Hanseniaspora and Starmerella genera).
- Assessment of probiotic properties (auto-aggregation, hydrophobicity, tolerance to bile and gastrointestinal fluids) and safety (hemolytic activity, antibiotic sensitivity).
Main Results:
- Fifteen anti-MRSA yeast strains were isolated, identified as Hanseniaspora (7) and Starmerella (8).
- Starmerella bacillaris CC-PT4 demonstrated excellent auto-aggregation, hydrophobicity, and tolerance to bile, low pH, and simulated gastrointestinal fluids.
- S. bacillaris CC-PT4 adapted to 37°C, showed no beta-hemolytic activity, and was sensitive to amphotericin B, indicating a good safety profile.
Conclusions:
- Starmerella bacillaris CC-PT4 is a promising candidate for a probiotic yeast with anti-MRSA properties.
- This study identifies a novel food-derived yeast with potential applications in managing MRSA gut colonization.
- Further research can explore S. bacillaris CC-PT4 as a functional food ingredient or therapeutic agent.
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