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Use of Human Perivascular Stem Cells for Bone Regeneration
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Bone regeneration in rat using polycaprolactone/gelatin/epinephrine scaffold.

Arian Ehterami1, Hossein Khastar2,3, Mostafa Soleimannejad4

  • 1Department of Mechanical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Drug Development and Industrial Pharmacy
|April 29, 2022
PubMed
Summary

This study developed a novel polycaprolactone/gelatin bone scaffold incorporating epinephrine for enhanced bone healing. The PCL/gelatin/EP1% scaffold demonstrated superior cell proliferation and bone regeneration in preclinical models.

Keywords:
Epinephrinebonegelatin nanofiberpolycaprolactonescaffold

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Bone healing relies on supportive structures like the extracellular matrix.
  • Scaffolds are crucial in bone tissue engineering for structural support.
  • Epinephrine (EP) has shown potential to enhance bone healing processes.

Purpose of the Study:

  • To develop a 3D polycaprolactone/gelatin (PCL/gelatin) bone scaffold using electrospinning and freeze-drying.
  • To investigate the effect of incorporating different concentrations of epinephrine (EP) on scaffold properties and bone healing.
  • To evaluate the biocompatibility and osteogenic potential of the fabricated scaffolds.

Main Methods:

  • Fabrication of 3D PCL/gelatin scaffolds via electrospinning and freeze-drying.
  • Incorporation of varying concentrations of epinephrine (EP).
  • Characterization included surface morphology, FTIR, porosity, mechanical strength, water contact angle, degradation rate, hemolysis, MTT assay, and in vivo rat calvaria defect model.

Main Results:

  • Scaffolds exhibited approximately 75% porosity.
  • EP addition decreased mechanical strength but increased degradation rate due to enhanced hydrophilicity.
  • In vitro and in vivo studies indicated optimal cell proliferation and bone healing in the PCL/gelatin/EP1% group.

Conclusions:

  • The developed PCL/gelatin/EP scaffold positively influences osteogenesis and bone healing.
  • The PCL/gelatin/EP1% scaffold shows significant potential for bone tissue engineering applications.
  • This scaffold holds promise for future clinical trials in bone regeneration.