Related Experiment Video
Updated: Sep 25, 2025

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
Published on: June 2, 2023
[Influence of N-alkynylaminosteroids on mitochondria function and autophagy in glioma cells]
J U Panada1, V A Klopava1, T A Kulahava2
1Research Institute for Physical Chemical Problems of the Belarusian State University, Minsk, Belarus.
Abstract:
In this work we examined the synthesized N-alkynyl-17-aminosteroids and N-alkynyl-20-aminosteroids (based on dehydroepiandrosterone and pregnenolone, respectively) for their effect on C6 rat glioma cell functions. At 10 μM, the compounds had an insignificant effect on C6 glioma mitochondrial membrane potential, but increased cell autophagy by 70-90%, comparable to the known autophagy inducer dexamethasone. Docking simulations predict a potential high-affinity interaction between N-alkynylaminosteroids and Keap1 and the Hedgehog pathway protein, Smoothened, which are involved in autophagy regulation. The possible mechanisms of observed processes are discussed.
More Related Videos
Related Concept Videos
Autophagy
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Drugs that Stabilize Microtubules
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Drugs that Destabilize Microtubules
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...

