Glow and Flash Adjustable Chemiluminescence with Tunable Waveband from the Same CuInS₂@ZnS Nanocrystal Luminophore

All commercial chemiluminescence (CL) assays are conducted with either glow or flash CL of eye-visible waveband from chemical luminophores. Herein, glow and flash, as well as waveband adjustable CL from the same nanoparticle luminophore of thiol-capped CuInS₂@ZnS nanocrystals (CIS@ZnS-Thiol), are proposed via extensively exploiting the differed redox nature of CL triggering reagents. Taking thiosalicylic acid (TSA) as the model thiol-capping agent, the electron-injection-initiated charge transfer between CIS@ZnS-TSA and reductant can bring out efficient glow CL while the hole-injection-initiated charge transfer between CIS@ZnS-TSA and oxidant can give off obvious flash CL under optimum conditions. The maximum emission wavelength for CL of CIS@ZnS-TSA is adjustable from 730 nm to 823 nm via employing different triggering agents. Promisingly, the coexistent reductant of N₂H₄·H₂O and oxidant of H₂O₂ can be employed as dual triggering reagents to trigger eye-visible and highly efficient flash CL from CIS@ZnS-TSA. The maximum emission intensity for flash CL of CIS@ZnS-TSA/N₂H₄-H₂O₂ is 101-fold greater than the glow CL of CIS@ZnS-TSA/N₂H₄ and 22-fold greater than the flash CL of CIS@ZnS-TSA/H₂O₂, respectively. The flash CL from CIS@ZnS-TSA/N₂H₄-H₂O₂ is qualified for highly sensitive and selective CL immunoassay in a commercialized typical procedure with the entire operating process manually terminated within 35 min.