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MicroRNA-873-5p suppresses cell malignant behaviors of thyroid cancer via targeting CXCL5 and regulating P53 pathway

Wang Chang1, Qing Chang1, Haodong Lu1

  • 1Department of Head and Neck Surgery, Tangshan Gongren Hospital, Tangshan, Hebei Province, China.

Human Vaccines & Immunotherapeutics
|April 29, 2022
PubMed

Insights

MicroRNA-873-5p inhibits thyroid cancer (TC) progression by targeting CXCL5 and the P53 pathway. This study identifies microRNA-873-5p as a potential biomarker for TC diagnosis and treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Thyroid cancer (TC) progression involves complex molecular mechanisms.
  • Identifying key regulatory factors like microRNAs and chemokines is crucial for understanding TC pathogenesis.

Purpose of the Study:

  • To investigate the roles of microRNA-873-5p and CXCL5 in thyroid cancer (TC) cells.
  • To elucidate the regulatory relationship between microRNA-873-5p, CXCL5, and the P53 pathway in TC.
  • To assess the potential of microRNA-873-5p as a diagnostic biomarker for TC.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for gene and microRNA expression analysis.
  • Western blot to assess protein expression and P53 pathway activation.
  • Bioinformatics analysis and dual-luciferase assay to predict and validate regulatory interactions.
  • In vitro cell experiments to evaluate the functional impact of microRNA-873-5p and CXCL5 on TC cell behavior.

Main Results:

  • CXCL5 expression was significantly upregulated in TC cells and tissues.
  • Downregulation of CXCL5 suppressed malignant behaviors of TC cells.
  • MicroRNA-873-5p suppressed CXCL5 expression, and this effect was reversed by CXCL5 overexpression.
  • MicroRNA-873-5p targeted CXCL5, modulated the P53 pathway, and inhibited TC cell malignancy.

Conclusions:

  • MicroRNA-873-5p exerts its anti-cancer effects in TC by targeting CXCL5 and influencing the P53 pathway.
  • The microRNA-873-5p/CXCL5/P53 axis represents a novel regulatory mechanism in thyroid cancer.
  • MicroRNA-873-5p holds promise as a potential biomarker for thyroid cancer.
Keywords:
CXCL5invasionmicroRNA-873-5pmigrationproliferationthyroid cancer

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