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Published on: May 12, 2020
Evaluation of antioxidants, nitrosative, and oxidative stress before & after acute brucellosis treatment
Mehdi Gholami Bahnemiri1, Soleiman Mahjoub2, Mohammad Reza Hasanjani Roshan3
1Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Islamic Republic of Iran; Student Research Committee, Babol University of Medical Sciences, Babol, Islamic Republic of Iran; Department of Clinical Biochemistry, School of Medicine, Babol University of Medical Sciences, Babol, Islamic Republic of Iran.
Background:
In vivo studies of antioxidants, nitrosative, and oxidative processes in Brucella infection have not been comprehensive. This research looked at these critical concerns before and after treating individuals with acute brucellosis.
Methods:
A total of 50 individuals with acute brucellosis were studied before and after treatment in Babol, Iran. Superoxide dismutase (SOD) activity, plasma total antioxidant capacity (TAC), catalase (CAT) activity, protein carbonyl, oxidative stress markers like malondialdehyde (MDA), and nitrosative stress markers such as nitrite oxide metabolites (NO3, NO2) were all assessed.
Results:
The individuals were 44.15 ± 13.5 years old on average. Before therapy, protein carbonyl and plasma MDA were 0.887 ± 0.12 and 0.725 ± 0.33, respectively, and after treatment, they were 0.697 ± 0.17 and 0.467 ± 0.48 (p < 0.05). After treatment, the average TAC level was greater (870.1 ± 88.64 mol/l vs. 670.13 ± 133.96 mol/l) (p < 0.001). Before and after therapy, CAT activity was significantly different (8.53 ± 2.70 vs. 6.94 ± 2.84, p < 0.001). In addition, SOD activity was 41.79 ± 9.79 before treatment and 28.95 ± 8.11 after treatment (p < 0.001). The difference in plasma nitrite oxide levels before and after therapy was substantial (50.89 ± 17.74 vs. 28.68 ± 15.97, p < 0.001).
Conclusion:
The findings revealed that in acute brucellosis, nitrosative and oxidative stress, as well as antioxidant depletion, were all elevated. The addition of appropriate natural antioxidants to therapy regimens may improve their effectiveness.
