Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ATM Deficiency Induces TGFβ-Mediated Stromal Programming in Pancreatic Cancer.

Cancer research·2026
Same author

Mechanical memory primes cells for confined migration.

Cell reports·2026
Same author

Local and sustainable production of biomaterials.

Nature materials·2025
Same author

Design of a Trigger-Responsive Photothermal Vesicle-Based Cargo Delivery Platform.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Isolation, Extraction, and Analysis of Cells After Confined Migration.

Current protocols·2025
Same author

The Rise of Mechanobiology for Advanced Cell Engineering and Manufacturing.

Advanced materials (Deerfield Beach, Fla.)·2025

Related Experiment Video

Updated: Sep 25, 2025

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
08:24

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

Published on: September 14, 2016

10.3K

Temperature-sensitive migration dynamics in neutrophil-differentiated HL-60 cells.

Galina Khachaturyan1,2, Andrew W Holle1,3,4, Karen Ende5

  • 1Department of Cellular Biophysics, Max Planck Institute for Medical Research, 69120, Heidelberg, Germany.

Scientific Reports
|April 29, 2022
PubMed
Summary

Inflammation triggers increased cell migration speed in neutrophils at higher temperatures. These cells also exhibit thermotaxis, migrating towards heat sources, enhancing their inflammatory response.

More Related Videos

Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration
06:42

Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration

Published on: May 24, 2024

1.7K
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
08:39

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Published on: June 1, 2010

46.6K

Related Experiment Videos

Last Updated: Sep 25, 2025

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
08:24

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

Published on: September 14, 2016

10.3K
Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration
06:42

Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration

Published on: May 24, 2024

1.7K
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
08:39

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Published on: June 1, 2010

46.6K

Area of Science:

  • Immunology
  • Cell Biology
  • Biophysics

Background:

  • Cell migration is crucial for wound healing and inflammation.
  • Neutrophils, or polymorphonuclear leukocytes (PMNs), are key responders to inflammation.
  • Tissue temperature increases during inflammation, potentially influencing neutrophil behavior.

Purpose of the Study:

  • To investigate the effect of temperature on neutrophil migration.
  • To analyze neutrophil response to temperature gradients (thermotaxis).
  • To understand how temperature influences neutrophil migration in simulated in vivo conditions.

Main Methods:

  • Utilized differentiated HL-60 cells as a neutrophil model.
  • Assessed migration speed, detachment, persistence time, and length at various temperatures (30-42°C).
  • Employed 2D and 3D collagen gradient chambers to study thermotaxis (27-43°C).

Main Results:

  • Neutrophil-like cell migration speed increased with temperature (30-42°C).
  • Higher temperatures led to increased cell detachment.
  • Cells demonstrated thermotaxis, migrating towards heat sources in both 2D and 3D environments.

Conclusions:

  • Elevated temperatures characteristic of inflammation enhance neutrophil migration speed and effectiveness.
  • Neutrophils can sense and respond to temperature gradients, exhibiting thermotaxis.
  • Findings suggest temperature plays a significant role in directing neutrophil migration during inflammatory responses.