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Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
Published on: September 14, 2016
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Temperature-sensitive migration dynamics in neutrophil-differentiated HL-60 cells
Galina Khachaturyan1,2, Andrew W Holle1,3,4, Karen Ende5
1Department of Cellular Biophysics, Max Planck Institute for Medical Research, 69120, Heidelberg, Germany.
Scientific Reports
|April 29, 2022
Summary
Inflammation triggers increased cell migration speed in neutrophils at higher temperatures. These cells also exhibit thermotaxis, migrating towards heat sources, enhancing their inflammatory response.
Area of Science:
- Immunology
- Cell Biology
- Biophysics
Background:
- Cell migration is crucial for wound healing and inflammation.
- Neutrophils, or polymorphonuclear leukocytes (PMNs), are key responders to inflammation.
- Tissue temperature increases during inflammation, potentially influencing neutrophil behavior.
Purpose of the Study:
- To investigate the effect of temperature on neutrophil migration.
- To analyze neutrophil response to temperature gradients (thermotaxis).
- To understand how temperature influences neutrophil migration in simulated in vivo conditions.
Main Methods:
- Utilized differentiated HL-60 cells as a neutrophil model.
- Assessed migration speed, detachment, persistence time, and length at various temperatures (30-42°C).
- Employed 2D and 3D collagen gradient chambers to study thermotaxis (27-43°C).
Main Results:
- Neutrophil-like cell migration speed increased with temperature (30-42°C).
- Higher temperatures led to increased cell detachment.
- Cells demonstrated thermotaxis, migrating towards heat sources in both 2D and 3D environments.
Conclusions:
- Elevated temperatures characteristic of inflammation enhance neutrophil migration speed and effectiveness.
- Neutrophils can sense and respond to temperature gradients, exhibiting thermotaxis.
- Findings suggest temperature plays a significant role in directing neutrophil migration during inflammatory responses.
