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Related Experiment Video

Updated: Sep 25, 2025

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion
08:54

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion

Published on: April 27, 2015

17.1K

Machine perfusion in liver transplantation.

Richard X Sousa Da Silva1,2, Achim Weber3, Philipp Dutkowski1

  • 1Department of Surgery and Transplantation, Swiss Hepato-Pancreato-Biliary and Transplant Center, University Hospital Zurich, Zurich, Switzerland.

Hepatology (Baltimore, Md.)
|April 30, 2022
PubMed
Summary
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Machine perfusion techniques are crucial for assessing marginal liver grafts, improving transplant outcomes. This review explores hypothermic and normothermic machine perfusion for enhanced organ viability and expanded donor criteria.

Area of Science:

  • Transplantation Medicine
  • Organ Preservation
  • Surgical Innovation

Background:

  • Liver transplantation faces a critical shortage of donor organs, leading to increased patient mortality.
  • Expanded donor criteria include marginal liver grafts (e.g., steatotic or from circulatory death donors), which carry higher risks.
  • Assessing the viability of these marginal grafts before transplantation is essential to mitigate complications.

Purpose of the Study:

  • To review current machine perfusion techniques for liver grafts.
  • To introduce a novel combined hypothermic and normothermic machine perfusion strategy.
  • To highlight the potential of ex situ treatment during perfusion to improve graft utilization and patient outcomes.

Main Methods:

  • Review of clinically applied hypothermic oxygenated perfusion (HOPE) and normothermic machine perfusion (NMP) techniques.

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Last Updated: Sep 25, 2025

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Published on: April 27, 2015

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  • Description of the mechanistic principles underlying these perfusion methods.
  • Presentation of a novel approach combining immediate organ assessment via HOPE with extended NMP and ex situ treatment.

    • Main Results:

    • Machine perfusion offers a method to assess and potentially improve marginal liver grafts.
    • The novel combined perfusion strategy allows for simultaneous organ assessment and ex situ treatment.
    • This approach has the potential to significantly increase the number of usable liver grafts.

    Conclusions:

    • Machine perfusion is a vital tool for evaluating and potentially enhancing marginal liver grafts.
    • A combined hypothermic and normothermic perfusion strategy with ex situ treatment offers a promising advancement.
    • This innovative approach could dramatically expand the donor liver pool and improve transplant success rates.