Oleamide as analyte protectant in GC analysis of THC and its metabolites in blood

Methods for the analysis of cannabinoids in bio-matrices are continually being developed, to achieve a proper sensitivity required for their detection and accuracy in their quantification. The presented paper shows that the analytical sensitivity of GC-MS to THC and its metabolites in blood samples can be significantly increase by oleamide (OLA) addition to the examined sample, which evokes the matrix effect of transient character. The magnitude of signal increment resulting from oleamide presence in the examined sample is the greatest for THC metabolites and depends on oleamide concentration in the examined sample. The use of transient matrix effect to increase the sensitivity of the analysis can be applied not only in QuEChERS procedure, which is applied in the described experiments, but also in other blood sample preparation methods. Evoking the transient matrix effect by means of OLA in the experimental analytical quantitation of THC and its metabolites in blood allowed to lower limit of detection (LOD) approximately by 20.5%, 87.6% and 90.1% in the case of THC, THC-OH and THC-COOH, respectively.