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LncRNA PART1 inhibits glioma proliferation and migration via miR-374b/SALL1 axis
Yong-Wen Deng1, Yu-Gao Shu1, Sheng-Li Sun1
1Department of Neurosurgery, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, 410005, Hunan Province, PR China.
Background:
Abnormal expression of lncRNA is involved in a diversity of diseases and plays a vital role in targeted therapy. However, few studies have been conducted on lncRNA PART1 in glioma. We aimed to investigate the function and the potential regulatory mechanism of lncRNA PART1/miR-374b/SALL1 axis in glioma.
Methods:
qRT-PCR and western blotting detected genes and proteins expression. Dual-luciferase reporter assay was performed to examine the binding relationship of lncRNA PART1, miR-374b, and SALL1. MTT assay and clone formation assay were performed to detect the cell viability and proliferation. Transwell assay detected glioma cell migration. In vivo tumor development experiments detected changes in tumor size, volume, and weight of the tumor after overexpression of lncRNA PART1. Immunohistochemistry was used to detect ki-67, E-cadherin, and N-cadherin expression.
Results:
The expression of lncRNA PART1 and SALL1 were down-regulated and miR-374b was up-regulated in different glioma cell lines. Overexpression of lncRNA PART1 inhibited glioma cell proliferation, migration, and epithelial mesenchymal transition (EMT). LncRNA PART1 targeted miR-374b to promote SALL1 expression. The knockdown of miR-374b inhibited glioma cell proliferation and migration and EMT by SALL1. What's more, overexpression of miR-374b or knockdown of SALL1 reversed the inhibitory effect of lncRNA PART1 on the proliferation, migration, and EMT of glioma cells. Furthermore, overexpression of lncRNA PART1 inhibited glioma growth in vivo.
Conclusion:
LncRNA PART1 inhibited glioma proliferation and migration via miR-374b/SALL1 axis. These results might provide new insights for comprehending the complex lncRNA-miRNA network in gliomas.
Insights
Long non-coding RNA PART1 (lncRNA PART1) inhibits glioma progression by regulating the miR-374b/SALL1 axis. This finding offers new insights into the lncRNA-miRNA network in glioma targeted therapy.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Abnormal long non-coding RNA (lncRNA) expression is implicated in various diseases, including cancer.
- lncRNAs play a crucial role in targeted cancer therapy.
- The specific role of lncRNA PART1 in glioma remains underexplored.
Purpose of the Study:
- To investigate the function of lncRNA PART1 in glioma.
- To elucidate the regulatory mechanism of the lncRNA PART1/miR-374b/SALL1 axis in glioma.
- To assess the therapeutic potential of targeting this axis in glioma.
Main Methods:
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting for gene and protein expression analysis.
- Dual-luciferase reporter assay to confirm interactions between lncRNA PART1, miR-374b, and SALL1.
- In vitro assays (MTT, clone formation, Transwell) and in vivo tumor xenograft models to evaluate glioma cell proliferation, migration, and tumor growth.
Main Results:
- lncRNA PART1 and SALL1 expression were downregulated, while miR-374b was upregulated in glioma cells.
- Overexpression of lncRNA PART1 suppressed glioma cell proliferation, migration, and epithelial-mesenchymal transition (EMT).
- lncRNA PART1 targets miR-374b, thereby promoting SALL1 expression, and this axis inhibits glioma growth in vivo.
Conclusions:
- lncRNA PART1 inhibits glioma proliferation and migration through the miR-374b/SALL1 pathway.
- This axis represents a potential therapeutic target for glioma.
- Findings contribute to understanding the complex lncRNA-miRNA regulatory networks in glioma.
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