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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
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Comprehensive analysis of DNA methylation for periodontitis
Zengbo Zhao1, Huimin Wang1, Xiaona Li1
1Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang, 050017, People's Republic of China.
International Journal of Implant Dentistry
|May 1, 2022
Summary
This study explored gene methylation in periodontitis, identifying 668 differentially methylated genes. Key genes involved in immune and inflammatory responses were highlighted, offering insights into periodontitis pathogenesis.
Area of Science:
- Genomics
- Epigenetics
- Oral Biology
Background:
- Periodontitis is an infectious disease and a risk factor for peri-implantitis, potentially leading to implant loss.
- DNA methylation plays a crucial role in the development and progression of inflammatory diseases.
- Limited research exists on gene methylation patterns in periodontitis.
Purpose of the Study:
- To investigate the gene methylation profiling in periodontitis.
- To identify differentially methylated genes (DMGs) associated with periodontitis.
- To explore the potential role of these genes in disease pathogenesis.
Main Methods:
- Utilized Gene Expression Omnibus (GEO) database, specifically dataset GSE173081, comprising 12 periodontitis and 12 normal samples.
- Analyzed methylation data to identify differentially methylated genes (DMGs).
- Performed Gene Ontology, pathway enrichment, and protein-protein interaction (PPI) network analyses to understand DMG functions and identify hub genes.
Main Results:
- Identified 668 DMGs, including 621 hypomethylated and 47 hypermethylated genes.
- Functional analysis revealed enrichment in intracellular signaling, cell components, cell-cell interaction, and cellular behaviors.
- Pathway analysis indicated enrichment in cGMP-PKG, RAF/MAP kinase, and PI3K-Akt signaling pathways for hypomethylated genes, and bacterial invasion and sphingolipid signaling for hypermethylated genes.
- Identified 10 hub genes (e.g., APP, PAX6, LPAR1, WNT3A) central to immune and inflammatory responses.
Conclusions:
- This study provides a comprehensive overview of gene methylation status in periodontitis.
- The identified DMGs and hub genes offer insights into the pathogenesis of periodontitis.
- Findings may guide future research and therapeutic strategies for periodontitis.
