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Related Experiment Video

Updated: Sep 25, 2025

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

Click Bioconjugation: Modifying Proteins Using Click-Like Chemistry.

Bernhard Stump¹

¹Lonza AG, Development Bioconjugates, Rottenstr. 6, 3930, Visp, Switzerland.

Chembiochem : a European Journal of Chemical Biology

|May 2, 2022

Summary

This summary is machine-generated.

This review explores click chemistry applications in bioconjugation for selective protein modification. It highlights successful reactions and new developments for bioorthogonal chemistry.

Keywords:

antibody drug conjugates bioconjugation bioorthogonal chemistry click chemistry

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Area of Science:

Biochemistry and Organic Chemistry

Background:

Bioconjugation involves chemically modifying proteins, requiring selective reactions.
Existing methods often need specific conditions or can interfere with biological functions.

Purpose of the Study:

To review the application of click chemistry in bioconjugation.
To showcase successful click-type reactions and recent advancements.
To address the need for selective, bioorthogonal modification of biomolecules.

Main Methods:

Review of literature on click chemistry and bioconjugation.
Analysis of reactions applicable in aqueous, mild pH conditions.
Discussion of bioorthogonal strategies for biomolecule modification.

Main Results:

Click chemistry offers highly selective and efficient transformations for bioconjugation.
Several click-type reactions have been successfully applied to protein modification.
New developments are expanding the toolbox for bioorthogonal chemistry.

Conclusions:

Click chemistry is a powerful tool for selective bioconjugation.
Continued development of bioorthogonal reactions is crucial for advanced biomolecule modification.