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Related Concept Videos

Chemical Agents for Microbial Control01:27

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Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
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Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
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Waterline Disinfectants Reduce Dental Bioaerosols: A Multitracer Validation.

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Dental unit waterline disinfectants significantly reduce viable virus dispersion in dental aerosols. This finding highlights their importance for infection control in dental settings.

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Area of Science:

  • Microbiology
  • Environmental Health
  • Infection Control

Background:

  • Dental procedures generate bioaerosols containing oral microbes.
  • Dental unit waterlines (DUWLs) are a potential source of contamination.
  • The impact of DUWL disinfectants on viral dispersion in aerosols remains understudied.

Purpose of the Study:

  • To investigate the effect of DUWL disinfectants on viral dispersion in dental bioaerosols.
  • To establish a dual-tracer system for evaluating disinfectant efficacy.
  • To assess the impact on viral viability and aerosol concentration.

Main Methods:

  • Utilized bacteriophage MS2 as a viral tracer and fluorescein as a fluorescent tracer.
  • Conducted simulated dental procedures on a mannequin using an air turbine handpiece.
  • Sampled aerosols and droplets, quantifying tracers via plaque assays, RT-qPCR, and fluorometry.
  • Assessed total aerosol concentration using an optical particle counter.

Main Results:

  • DUWL disinfectants reduced bacteriophage viability.
  • The disinfectant ICX substantially reduced bacteriophage detection (P < 0.001).
  • Fluorescein tracer results showed good agreement with bacteriophage tracer data.
  • Detected MS2 RNA in all samples, with some nonviable virions indicated by RT-qPCR positive/plaque assay negative results.

Conclusions:

  • DUWL disinfectants effectively reduce the dispersion of viable viruses in dental bioaerosols.
  • These disinfectants are crucial for routine infection control in dentistry.
  • Their use may mitigate risks during infectious disease outbreaks.