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CyTOF® for the Masses.

Akshay Iyer1,2, Anouk A J Hamers1,2,3, Asha B Pillai1,2,3,4

  • 1Department of Pediatrics, University of Miami Miller School of Medicine, Miami, FL, United States.

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|May 2, 2022
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Summary
This summary is machine-generated.

Mass cytometry offers deep immunophenotyping for complex samples like tumor immunotherapy. This guide simplifies mass cytometry experiment design, acquisition, and analysis for researchers new to the technique.

Keywords:
CyTOFbarcodingdata analysisexperimental designmass cytometrypanel designprotocolworkflow

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Area of Science:

  • Immunology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Mass cytometry revolutionizes immunophenotyping, enabling deep characterization of immune cells.
  • It excels in analyzing limited samples, crucial for preclinical and clinical tumor immunotherapy research.
  • By using metal isotopes instead of fluorophores, it overcomes spectral overlap issues inherent in optical flow cytometry.

Purpose of the Study:

  • To provide a comprehensive resource guide for mass cytometry.
  • To address challenges in experiment design, execution, and data analysis for novice users.
  • To streamline mass cytometry workflows for researchers transitioning from flow cytometry.

Main Methods:

  • The guide covers critical aspects including experiment and panel design.
  • It details antibody conjugation, sample staining, and acquisition protocols.
  • Data pre-processing and analysis strategies are thoroughly explained, with comparisons of available resources.

Main Results:

  • The resource guide offers a structured approach to mass cytometry.
  • It facilitates the development of data-driven project workflows.
  • It aims to empower both novice and advanced users in leveraging mass cytometry.

Conclusions:

  • This guide serves as a valuable resource for mass cytometry users.
  • It simplifies complex protocols, enhancing experimental reproducibility.
  • It supports broader adoption and effective utilization of mass cytometry in immunological research.