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Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
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Related Experiment Video

Updated: Sep 25, 2025

Construction of a Low-cost Mobile Incubator for Field and Laboratory Use
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OpenTCC: An open source low-cost temperature-control chamber.

Carlos Sánchez1, Paolo Dessì1, Maeve Duffy2

  • 1Microbiology Department, School of Natural Sciences, National University of Ireland, Galway, University Road, Galway, Ireland.

Hardwarex
|May 2, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed an open-source temperature-control chamber (OpenTCC) for microbial electrochemical technologies (MET). This low-cost system offers stable temperature control and reduced electromagnetic interference for environmental applications.

Keywords:
BioelectrochemistryElectrical noiseFaraday cageIncubatorPeltier element

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Area of Science:

  • Environmental Science
  • Electrochemistry
  • Biotechnology

Background:

  • Microbial electrochemical technologies (MET) are crucial for renewable energy and pollution remediation.
  • Stable temperatures and low electromagnetic interference are essential for reliable MET research.
  • Existing heating methods pose safety risks or interfere with electrochemical analyses.

Purpose of the Study:

  • To introduce a low-cost, open-source temperature-control chamber (OpenTCC) for MET.
  • To provide stable temperature control (20-55°C) with reduced electromagnetic interference.
  • To offer a flexible and adaptable platform for laboratory applications.

Main Methods:

  • The OpenTCC utilizes a lightweight structure with heating pads and Peltier-cooling modules.
  • Open-source electronic circuits, an Arduino microcontroller, and a Python interface control the hardware.
  • The system features modularity for stacking and includes data-logging capabilities.

Main Results:

  • The OpenTCC successfully provides reliable and tunable temperature control within the specified range.
  • It significantly reduces electromagnetic interference compared to conventional methods.
  • The system is more cost-effective than commercial temperature controllers.

Conclusions:

  • The OpenTCC is a viable, affordable solution for temperature control in MET research.
  • Its adaptable design makes it suitable for various laboratory applications beyond MET.
  • The open-source nature facilitates customization and future upgrades.