Effects of Maren Pills on the Intestinal Microflora and Short-Chain Fatty Acid Profile in Drug-Induced Slow Transit...

Area of Science:

• Gastroenterology
• Pharmacology
• Microbiology

Background:

• Slow transit constipation (STC) is a prevalent condition requiring safe and effective treatments.
• Investigating novel therapeutic approaches for STC is crucial for public health.

Purpose of the Study:

• To evaluate the laxative effects of Maren pills (MRW) in a rat model of STC.
• To elucidate the mechanisms underlying MRW's efficacy, focusing on intestinal microflora, metabolism, and the 5-HT signaling pathway.

Main Methods:

• Loperamide hydrochloride was used to induce STC in rats.
• MRW was administered intragastrically for two weeks.
• Intestinal microflora, short-chain fatty acids (SCFAs), and the 5-HT signaling pathway were analyzed using 16s rDNA sequencing, HPLC, and molecular biology techniques (WB, ELISA, immunofluorescence, immunohistochemistry).

Main Results:

• MRW treatment significantly improved STC symptoms, including increased fecal output, water content, and intestinal propulsion.
• MRW modulated the colonic microflora, increasing beneficial *Lactobacillus* and decreasing *Prevotella*.
• MRW enhanced SCFA production (acetic, propionic, butyric acids) and activated the colonic 5-HT pathway.

Conclusions:

• Maren pills (MRW) demonstrate significant therapeutic potential for slow transit constipation (STC).
• The mechanism involves regulating intestinal microflora composition and enhancing SCFA metabolism.
• MRW's efficacy is linked to the modulation of the 5-HT signaling pathway.