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Updated: Sep 25, 2025

Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay
Published on: May 5, 2016
Benzimidazolium salts prevent and disrupt methicillin-resistant Staphylococcus aureus biofilms
Jérémie Tessier1, Andreea R Schmitzer1
1Department of Chemistry, University of Montreal PO Box 6128, Succursale Centre-Ville Montreal QC H3C 3J7 Canada ar.schmitzer@umontreal.ca.
Abstract:
Emergence of resistant bacteria encourages us to develop new antibiotics and strategies to compensate for the different mechanisms of resistance they acquire. One of the defense mechanisms of resistant bacteria is the formation of biofilms. Herein we show that benzimidazolium salts with various flexible or rigid side chains act as strong antibiotic and antibiofilm agents. We show that their antibiofilm activity is due to their capacity to destroy the biofilm matrix and the bacterial cellular membranes. These compounds are able to avoid the formation of biofilms and disperse mature biofilms showing a universal use in the treatment of biofilm-associated infections.
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