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Updated: Sep 25, 2025
Author Spotlight: Accelerating Discovery in Microporous Material Chemistry
Published on: October 6, 2023
Characterization of D-Allulose-3-Epimerase From Ruminiclostridium papyrosolvens and Immobilization Within
Jiaming Yang1, Dexun Fan1, Fengguang Zhao2
1Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.
Abstract:
D-allulose is one sort of C-3 epimer of D-fructose with the low calorie (0.4 kcal/g) and high sweetness (70% of the relative sweetness of sucrose), which can be biosynthesized by D-allulose-3-epimerase (DAE). In this work, we report the characterization of a novel DAE from Ruminiclostridium papyrosolvens (RpDAE) by genome mining approach. The activity of RpDAE reached maximum at pH 7.5 and 60°C, supplemented with 1 mM Co2+. Using D-fructose (500 g/L) as the substrate for epimerization reaction, RpDAE produced D-allulose (149.5 g/L). In addition, RpDAE was immobilized within the microporous zeolite imidazolate framework, ZIF67, by in situ encapsulation at room temperature. The synthesized bio-composites were characterized by powder X-ray diffraction and Fourier transform infrared spectroscopy. RpDAE-ZIF67 maintained 56% of residual activity after five reaction cycles. This study provides helpful guidance for further engineering applications and industrial production of D-allulose.
