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Area of Science:

  • Analytical Chemistry
  • Nanomaterials Science

Background:

  • Metal nanoclusters (NCs) are emerging luminescent nanomaterials with significant potential in analytical chemistry.
  • Palladium nanoclusters (Pd NCs) offer unique optical properties for sensing applications.

Purpose of the Study:

  • To synthesize and characterize polyethyleneimine-stabilized palladium nanoclusters (PEI-Pd NCs).
  • To develop a novel fluorescent nanosensor for the detection of oxytetracycline (OTC) using PEI-Pd NCs.

Main Methods:

  • Synthesis of PEI-Pd NCs via chemical reduction.
  • Characterization of spectral properties, surface structure, and morphology.
  • Investigation of nanosensor selectivity, stability, and photobleaching resistance.
  • Quantitative analysis of OTC using fluorescence quenching and inner filter effect (IFE).

Main Results:

  • PEI-Pd NCs demonstrated good biocompatibility, stability, and photobleaching resistance in aqueous solutions.
  • A good linear relationship was observed between fluorescence quenching and OTC concentration (25-440 nM, R²=0.99).
  • The limit of detection (LOD) for OTC was determined to be 22 nM.

Conclusions:

  • A new nanosensing analysis method for OTC detection was successfully established.
  • The PEI-Pd NCs-based fluorescent nanosensor offers a sensitive and reliable approach for OTC determination.
  • The inner filter effect (IFE) mechanism plays a key role in the sensing process.