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Updated: Sep 25, 2025

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

The FUSION protein crystallization screen.

Fabrice Gorrec¹, Dom Bellini¹

¹Structural Studies, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, Cambridgeshire CB2 0QH, United Kingdom.

Journal of Applied Crystallography

|May 2, 2022

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A new protein crystallization screen, FUSION, streamlines atomic structure determination by efficiently producing diffraction-quality crystals. This innovative formulation accelerates research by reducing sample and time consumption in structural biology.

Area of Science:

Structural Biology
Biophysics
Biochemistry

Background:

X-ray crystallography requires high-quality crystals for atomic structure determination of biological macromolecules.
Screening crystallization conditions is time-consuming and sample-intensive.
Existing screening methods can be inefficient, hindering rapid structural analysis.

Purpose of the Study:

To develop an innovative protein crystallization screen formulation to improve crystal production efficiency.
To reduce the time and sample required for screening crystallization conditions.
To facilitate faster atomic structure determination of biological macromolecules.

Main Methods:

Developed a novel protein crystallization screen formulation named FUSION.

Keywords:

X-ray crystallography crystallization screens macromolecular crystallization protein crystals

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Last Updated: Sep 25, 2025

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

Published on: January 9, 2012

Crystallization of Membrane Proteins in Lipidic Mesophases

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

Combined efficient components from three MORPHEUS screens using a systematic approach.

Integrated 96 unique combinations of crystallization additives, including small molecules and ions found in the Protein Data Bank (PDB).

Main Results:

FUSION demonstrated high yields of diffraction-quality crystals for seven different test proteins.
The screen significantly reduced the time and sample needed for crystallization screening.
Two novel crystal forms for α-amylase and avidin were discovered, not previously present in the PDB.

Conclusions:

FUSION is an effective and efficient tool for producing diffraction-quality protein crystals.
This formulation accelerates the process of atomic structure determination by X-ray crystallography.
FUSION advances structural biology research by enabling faster and more economical crystal screening.