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Final irrigation protocols affect radicular dentin DMP1-CT expression, microhardness, and biochemical composition.
Cristina Retana-Lobo1, Tatiana Ramírez-Mora1, Fabian Murillo-Gómez2
1Department of Endodontics, Faculty of Dentistry, LICIFO, University of Costa Rica, SJO, Sabanilla, San José, 11502, Costa Rica.
Clinical Oral Investigations
|May 2, 2022
Summary
Final irrigation protocols using sodium hypochlorite and passive ultrasonic irrigation negatively impact radicular dentin. These methods reduce microhardness and alter biochemical composition, potentially affecting dental regeneration.
Area of Science:
- Endodontics
- Dental Materials Science
- Biomaterials
Background:
- Radicular dentin properties are crucial for successful endodontic treatment outcomes.
- Understanding the impact of irrigation protocols on dentin is essential for preserving tooth structure.
Purpose of the Study:
- To assess how different final irrigation protocols affect radicular dentin's microhardness, biochemical makeup, and DMP1-CT expression.
- To compare the effects of various irrigation solutions and techniques on dentin integrity.
Main Methods:
- 140 human teeth underwent preparation and were divided into 7 irrigation groups.
- Dentin microhardness, DMP1-CT expression, ultrastructure, and biochemical composition (calcium, phosphorus) were analyzed.
- Techniques included Vickers hardness testing, immunohistochemistry, Raman spectroscopy, EDAX, and SEM.
Main Results:
- Protocols using sodium hypochlorite (NaOCl) and passive ultrasonic irrigation (PUI) showed reduced DMP1-CT expression and lower calcium/phosphorus content.
- SEM revealed dentin erosion and permeable tubules, especially with PUI.
- NaOCl-EDTA irrigation maintained higher microhardness compared to PUI:NaOCl-EDTA-NaOCl, which had the lowest values.
Conclusions:
- Final irrigation with NaOCl and PUI adversely affects radicular dentin's DMP1-CT expression, biochemical composition, and microhardness.
- Optimized irrigation protocols are vital for preserving dentin, promoting adhesion, and supporting biomineralization and regeneration.
