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Protein Complexes with Interchangeable Parts

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Related Experiment Video

Updated: Sep 24, 2025

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

Y-box Binding Protein 1: Looking Back to the Future.

Valentina Evdokimova¹

¹Ontario Institute for Cancer Research, Toronto, ON, Canada. v.evdokimova@gmail.com.

Biochemistry. Biokhimiia

|May 3, 2022

Summary

This summary is machine-generated.

Y-box binding protein 1 (YB-1) is crucial for normal cell mRNA biogenesis and function. Understanding its role in healthy cells is key to deciphering its pathological functions in diseases like cancer.

Keywords:

YB-1 YBX1 cancer cold-shock proteins gene expression mRNA mRNP regulation stress adaptation translation

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Last Updated: Sep 24, 2025

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

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Published on: May 17, 2016

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Published on: January 20, 2019

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Published on: October 2, 2017

Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

Y-box binding protein 1 (YB-1) is a cold shock domain (CSD) protein implicated in numerous human diseases.
YB-1 and related CSD proteins are highly conserved and ubiquitously expressed, playing vital roles in cellular physiology.
These proteins are essential for coordinating all aspects of mRNA biogenesis and function.

Purpose of the Study:

To critically reassess the pathological functions attributed to YB-1.
To highlight the fundamental roles of YB-1 and CSD proteins in normal cellular processes.
To establish a foundation for understanding YB-1's aberrant functions in diseases, particularly cancer.

Main Methods:

Literature review and critical analysis of existing research on YB-1 and CSD proteins.
Synthesis of data on the physiological functions of YB-1 in various cellular contexts.
Examination of the evolutionary conservation and expression patterns of YB-1.

Main Results:

YB-1 is integral to mRNA transcription, translation, storage, and degradation.
Its functions are critical during cellular processes requiring rapid gene expression changes, such as development and stress response.
Understanding YB-1's normal functions provides context for its dysregulation in pathological conditions.

Conclusions:

A comprehensive understanding of YB-1's basic properties and functions in normal cells is essential.
This knowledge is critical for elucidating YB-1's precise role in tumorigenesis and other complex diseases.
Further research into YB-1's normal cellular roles will illuminate its pathological contributions.