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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Related Experiment Video

Updated: Sep 24, 2025

Transcriptome Analysis of Single Cells
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Advanced tools and methods for single-cell surgery.

Adnan Shakoor1, Wendi Gao2, Libo Zhao2

  • 1Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China.

Microsystems & Nanoengineering
|May 3, 2022
PubMed
Summary
This summary is machine-generated.

Developing precise micromanipulation tools is crucial for advancing cell-based therapies and understanding diseases. This review details laser, microneedle, and untethered micro/nanotools for single-cell surgery, discussing their limitations and applications.

Keywords:
EngineeringNanobiotechnology

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Medical Engineering

Background:

  • Advanced cell manipulation is essential for developing novel cell-based therapies.
  • Understanding cell dynamics, functions, and disease mechanisms requires precise tools.
  • Single-cell surgery offers a pathway to detailed biological investigation.

Purpose of the Study:

  • To review micro/nanomanipulation tools and control methods for single-cell surgery.
  • To detail specific micromanipulation techniques, including laser, microneedle, and untethered tools.
  • To discuss the limitations and biological/clinical significance of single-cell surgery.

Main Methods:

  • Literature review of micro/nanomanipulation techniques.
  • Detailed presentation of laser-based micromanipulation.
  • In-depth analysis of microneedle and untethered micro/nanotool methods.

Main Results:

  • Identification and categorization of key micromanipulation tools.
  • Discussion of the operational principles and limitations of each technique.
  • Exploration of the biological significance and clinical potential.

Conclusions:

  • Precise micromanipulation tools are vital for cell-based medical advancements.
  • Laser, microneedle, and untethered tools offer distinct approaches to single-cell surgery.
  • Further development is needed to overcome current limitations and expand clinical applications.