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[Transcriptome analysis in fetal lungs of SRC1/SRC2 double-knockout mice].

Ya-Qin Yu1, Huai-Yan Chen1, Yuan-Yuan Liu1

  • 1Department of Physiology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, China.

Sheng Li Xue Bao : [Acta Physiologica Sinica]
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Summary

Steroid receptor coactivators (SRCs) are crucial for fetal lung development. SRC1/2 double-knockout mice exhibit altered gene expression in fetal lungs, impacting development and potentially labor initiation.

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Area of Science:

  • Genomics
  • Developmental Biology
  • Molecular Endocrinology

Background:

  • Steroid receptor coactivators (SRCs) regulate gene transcription and are vital for physiological functions.
  • Previous studies linked SRC1/2 double-knockout (dKO) fetuses to delayed labor, possibly due to lung hypoplasia and reduced surfactant secretion.
  • A comprehensive transcriptome-level analysis of SRC1/2 dKO fetal lungs was lacking.

Purpose of the Study:

  • To systematically analyze gene expression changes in fetal lungs of SRC1/2 dKO mice at the whole transcriptome level.
  • To identify differentially expressed genes (DEGs) and enriched pathways in SRC1/2 dKO fetal lungs.
  • To provide insights into the role of SRCs in fetal lung development and labor initiation.

Main Methods:

  • Transcriptome mRNA sequencing of fetal lung samples from SRC1/2 dKO and wild-type (WT) mice at 18.5 days post coitus.
  • Differential gene expression analysis (DEGs) between various knockout genotypes and WT.
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis for DEGs.

Main Results:

  • High-quality sequencing data (>92% Q30 score) confirmed experimental reliability.
  • SRC1/2 dKO fetal lungs showed significant DEGs compared to WT, SRC1KO, and SRC2KO.
  • DEGs were enriched in extracellular components, multicellular organ development, complement system, and extracellular matrix-receptor interactions.

Conclusions:

  • This study provides a comprehensive transcriptomic profile of SRC1/2 dKO fetal lungs.
  • Identified DEGs and pathways offer new theoretical basis for understanding fetal lung development regulation.
  • Findings suggest a role for SRCs in fetal lung development and potential influence on labor initiation signals.