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Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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Related Experiment Video

Updated: Sep 24, 2025

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
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Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

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A new reliable method for tissue preservation.

Elisabetta Cavalcanti1, Maria Scaramuzzi2, Raffaele Armentano1

  • 1Histopathology Unit of National Institute of Gastroenterology "S. de Bellis," Research Hospital Castellana Grotte Bari, Italy.

Pathology, Research and Practice
|May 3, 2022
PubMed
Summary
This summary is machine-generated.

A novel tissue preservation method offers a safe and effective alternative to formalin fixation and paraffin embedding (FFPE). This innovative technique maintains tissue integrity for diverse analyses, including histology and genetic testing, without toxic procedures.

Keywords:
Dried/rehydrated tissuesFFPETissue preservation

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Area of Science:

  • Histopathology
  • Pathology
  • Biotechnology

Background:

  • Tissue preservation is crucial for histological assays, but current methods like formalin fixation and paraffin embedding (FFPE) have limitations.
  • FFPE is carcinogenic and degrades nucleic acids, while other methods like plastination restrict further analysis.
  • Developing advanced preanalytical tools for high-quality tissue preservation remains a significant challenge.

Purpose of the Study:

  • To introduce and evaluate a new, patent-protected method for preserving biological tissues.
  • To assess the method's efficacy in maintaining nucleic acid and protein integrity for various downstream applications.
  • To provide a safe, cost-effective, and locally available alternative to traditional tissue preservation techniques.

Main Methods:

  • The study describes a new tissue preservation technique applicable to post-surgical or endoscopic specimens.
  • The method allows tissues to be restored to a pre-drying state for further processing.
  • This patented process (EP 18185364.9A) ensures preservation for histology, cytology, immunology, and genetic analyses.

Main Results:

  • The new method successfully preserves tissues for histopathological examination, tissue banking, and immunohistochemistry.
  • It maintains the integrity of nucleic acids and proteins.
  • The technique enables further dissection and histopathologically based studies, overcoming limitations of current methods.

Conclusions:

  • This innovative tissue preservation method offers a viable alternative to FFPE, addressing its toxicity and nucleic acid degradation issues.
  • The process is cost-effective, non-toxic, and suitable for local application in pathology institutes.
  • It meets the growing demand for reliable tissue preservation in histology, molecular diagnostics, and forensic medicine.