Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.3K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

14.1K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
14.1K
Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

1.1K
In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
1.1K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.2K
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exercise Resistance in Obese Male NZO Mice Manifests as Local Muscle Remodelling Without Glycaemic Improvements.

Diabetes, obesity & metabolism·2026
Same author

A biallelic MRPL42 variant causes a combined oxidative phosphorylation deficiency syndrome revealed by multi-omics.

NPJ genomic medicine·2026
Same author

Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy.

Cell·2026
Same author

Nuclear speckles enable processing of RNA from GC-rich isochores.

Cell·2026
Same author

Effects of hypothermic oxygenated machine perfusion on bile composition after liver transplantation - Findings from a randomized controlled trial.

JHEP reports : innovation in hepatology·2026
Same author

Multiomics approach identifies SERPINB1 as candidate biomarker for spinocerebellar ataxia type 2.

Scientific reports·2025

Related Experiment Video

Updated: Sep 24, 2025

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
12:01

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Published on: January 12, 2015

10.3K

Sleep neuron depolarization promotes protective gene expression changes and FOXO activation.

Anastasios Koutsoumparis1, Luisa M Welp2, Alexander Wulf2

  • 1Chair of Cellular Circuits and Systems, Biotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technical University Dresden, 01307 Dresden, Germany.

Current Biology : CB
|May 3, 2022
PubMed
Summary

Sleep-active neurons promote protective gene expression during normal sleep. Disturbing sleep increases this neuron activity, further boosting protective gene expression as a safeguarding response.

Keywords:
C. elegansDAF-16FoxORIS neurondevelopmental arrestgene expressionoptogeneticssleepsleep deprivationsleep-active neuron

More Related Videos

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

1.1K
Fluorescence Activated Cell Sorting FACS and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
08:37

Fluorescence Activated Cell Sorting FACS and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

Published on: August 27, 2016

29.6K

Related Experiment Videos

Last Updated: Sep 24, 2025

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
12:01

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Published on: January 12, 2015

10.3K
Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

1.1K
Fluorescence Activated Cell Sorting FACS and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
08:37

Fluorescence Activated Cell Sorting FACS and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

Published on: August 27, 2016

29.6K

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Sleep is vital for recuperation and survival, involving complex gene expression regulation.
  • Sleep deprivation induces stress responses and alters gene expression, but the mechanisms remain unclear.
  • Sleep-active neurons, like the RIS neuron in C. elegans, are crucial for initiating sleep and promoting survival.

Purpose of the Study:

  • To investigate the role of sleep-active neuron depolarization in regulating protective gene expression during normal and disturbed sleep.
  • To elucidate the molecular pathways linking sleep-active neurons to stress response genes.

Main Methods:

  • Utilized the nematode C. elegans as a model organism.
  • Examined the depolarization of the sleep-active RIS neuron.
  • Analyzed the expression of protective genes, including DAF-16 target genes like HSP-12.6, using molecular techniques.
  • Investigated the effects of mechanical stimulation on sleep and RIS neuron activity.

Main Results:

  • RIS neuron depolarization promotes protective gene expression, including the activation of FOXO/DAF-16 and its target genes.
  • Mechanical stimulation, which disturbs sleep, increases RIS neuron depolarization.
  • Activated RIS neurons lead to the upregulation of DAF-16 and other survival-associated genes.
  • Both normal sleep and sleep disturbance involve RIS depolarization, with disturbance leading to heightened activation.

Conclusions:

  • Sleep-active neuron depolarization is directly linked to the induction of protective gene expression.
  • During normal sleep, RIS depolarization facilitates a baseline protective response.
  • Sleep deprivation exacerbates RIS depolarization, leading to an amplified protective gene expression.
  • The cellular stress response to sleep deprivation can be viewed as a consequence of overactivated sleep-active neurons, acting as a protective mechanism.