Related Experiment Video
Updated: Sep 24, 2025

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor
Published on: March 22, 2024
Extracellular vesicle-induced cyclic AMP signaling
Aritra Bhadra1, April K Scruggs1, Silas J Leavesley2
1Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL, United States of America; Center for Lung Biology, College of Medicine, University of South Alabama, Mobile, Alabama.
Abstract:
Second messenger signaling is required for cellular processes. We previously reported that extracellular vesicles (EVs) from stimulated cultured endothelial cells contain the biochemical second messenger, cAMP. In the current study, we sought to determine whether cAMP-enriched EVs induce second messenger signaling pathways in naïve recipient cells. Our results indicate that cAMP-enriched EVs increase cAMP content sufficient to stimulate PKA activity. The implications of our work are that EVs represent a novel intercellular mechanism for second messenger, specifically cAMP, signaling.
More Related Videos
Related Concept Videos
Intracellular Signaling Cascades
Amplifying Signals via Second Messengers
cAMP-dependent Protein Kinase Pathways
What are Second Messengers?
IP3/DAG Signaling Pathway
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...

