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Extracellular vesicle-induced cyclic AMP signaling.

Aritra Bhadra1, April K Scruggs1, Silas J Leavesley2

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Cellular Signalling
|May 3, 2022
PubMed
Summary

Extracellular vesicles (EVs) carrying cyclic adenosine monophosphate (cAMP) can activate signaling pathways in recipient cells. This study shows EVs provide a novel way for cells to communicate using cAMP second messenger signaling.

Keywords:
Cyclic adenosine monophosphateExtracellular vesiclesMicrovesicleSecond messenger

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Area of Science:

  • Cellular biology
  • Molecular signaling
  • Biochemistry

Background:

  • Second messenger signaling is crucial for cellular functions.
  • Extracellular vesicles (EVs) derived from stimulated endothelial cells were previously found to contain cyclic adenosine monophosphate (cAMP).

Purpose of the Study:

  • To investigate if cAMP-enriched EVs can initiate second messenger signaling in recipient cells.
  • To determine the functional impact of EV-mediated cAMP transfer.

Main Methods:

  • Isolation and characterization of cAMP-enriched EVs from stimulated endothelial cells.
  • Incubation of naïve recipient cells with these EVs.
  • Measurement of intracellular cAMP levels and protein kinase A (PKA) activity in recipient cells.

Main Results:

  • Exposure to cAMP-enriched EVs led to increased intracellular cAMP levels in recipient cells.
  • The elevated cAMP levels were sufficient to activate protein kinase A (PKA) signaling.
  • This demonstrates functional transfer of a second messenger via EVs.

Conclusions:

  • Extracellular vesicles serve as a novel mechanism for intercellular communication.
  • EVs can effectively transfer cyclic adenosine monophosphate (cAMP) to recipient cells, inducing downstream signaling.
  • This finding opens new avenues for understanding cell-to-cell communication and potential therapeutic strategies involving second messengers.