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Updated: Sep 24, 2025

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
Published on: November 4, 2019
GFI1 regulates chromatin state essential in human endothelial-to-haematopoietic transition
Baoqiang Kang1,2,3, Tian Zhang2,3,4,5,6, Ke Huang2,3,4,7
1Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, China.
Objectives:
During embryonic haematopoiesis, haematopoietic stem/progenitor cells (HSPCs) develop from hemogenic endothelial cells (HECs) though endothelial to haematopoietic transition (EHT). However, little is known about how EHT is regulated in human. Here, we report that GFI1 plays an essential role in enabling normal EHT during haematopoietic differentiation of human embryonic stem cells (hESCs).
Results:
GFI1 deletion in hESCs leads to a complete EHT defect due to a closed chromatin state of hematopoietic genes in HECs. Mechanically, directly regulates important signaling pathways essential for the EHT such as PI3K signaling.etc.
Conclutions:
Together, our findings reveal an essential role of GFI1 mediated epigenetic mechanism underlying human EHT during hematopoiesis.
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